Capabilities/Cryo-Electron Microscopy

Cryo-Electron Microscopy

We support the full cryo-EM workflow for structural biology, soft matter, and nanomaterials research — from sample vitrification to near-atomic resolution structure determination.

Cryo-Transmission Electron Microscopy

FEI Titan Krios G3i Cryo-TEM

A state-of-the-art 300 kV cryo-TEM equipped with a Gatan K3 direct electron detector and BioQuantum energy filter. Designed for high-resolution single-particle analysis and cryo-electron tomography.

Accelerating Voltage300 kV
Resolution< 2 Å (single-particle SPA)
DetectorGatan K3 direct electron detector
Best ForHigh-res SPA, cryo-ET of macromolecules

FEI Talos Arctica Cryo-TEM

A 200 kV cryo-TEM optimized for high-throughput single-particle cryo-EM data collection with automated sample screening.

Accelerating Voltage200 kV
Resolution2–4 Å (SPA, sample-dependent)
DetectorFalcon 3EC direct electron detector
Autoloader12-cassette automated sample loading

FEI Glacios Cryo-TEM

A compact 200 kV cryo-TEM for routine sample screening and initial quality assessment before high-resolution data collection.

Accelerating Voltage200 kV
Resolution3–6 Å (SPA screening)
DetectorFalcon 3 direct electron detector
Best ForSample screening, preliminary data collection

Sample Preparation

Vitrobot Mark IV Cryo-Plunger

An automated vitrification system for preparing cryo-EM specimens with precise control over blotting force, time, and humidity.

Humidity Control0–100% RH
Temperature Control4–60°C (chamber)
Blot Force0–20 (arbitrary units, adjustable)
Grid TypesLacey, holey carbon, UltrAuFoil, Quantifoil

Leica EM GP2 Cryo-Plunger

A grid preparation system for cryo-EM enabling plunge-freezing of biological samples in liquid ethane with controlled humidity.

Humidity ControlUp to 99% RH
Temperature Control4–50°C (chamber)
CryogenLiquid ethane or ethane/propane mix
Grid TypesStandard 3 mm TEM grids

Leica EM ACE600 Cryo-Coater

A high-vacuum sputter coater for cryo-SEM sample preparation. Applies ultra-thin conductive coatings at cryogenic temperatures.

Coating MaterialsPt, Ir, W, Au/Pd
Coating Thickness1–10 nm (controlled)
TemperatureDown to −120°C (cryo stage)
Best ForCryo-SEM of frozen-hydrated samples

Cryo-FIB Sample Preparation

FEI Helios NanoLab 600i Cryo-FIB-SEM

A dual-beam cryo-FIB-SEM for preparing cryo-lamellae from frozen-hydrated biological samples for cryo-ET.

FIB BeamGa⁺, 1 pA – 65 nA
Lamella Thickness50–200 nm (typical)
Cryo Stage Temp.Down to −185°C
Best ForIn-situ cryo-lamella preparation for cryo-ET

Data Processing & Analysis

Single-Particle Analysis (SPA)

Computational workflow for determining 3D structures of macromolecular complexes from thousands of 2D cryo-EM particle images.

Achievable Resolution2–4 Å (well-behaved samples)
Particle Size Range100 kDa – MDa complexes
SoftwareRELION, cryoSPARC, CTFFIND
Output3D density map, atomic model

Cryo-Electron Tomography (cryo-ET)

A technique for 3D imaging of unique, pleomorphic structures in their native cellular context via tilt-series reconstruction.

Resolution2–5 nm (subtomogram averaging)
Tilt Range±70° (typical)
Sample Thickness< 300 nm (optimal)
SoftwareIMOD, RELION, Dynamo

Need Cryo-EM Services?

Contact us to discuss your structural biology or soft matter characterization needs.